Terminator

Part:BBa_K4687049:Design

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-12)


T1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CRISPR-MAD7 nuclease has been described as targeting a wider range of PAM sequences, i.e. 5'-YTTN-3', and has demonstrated high gene editing activity in microbial systems, with small molecular weights, and a very wide range of applications. Then in the process of its use, when the bacteria can carry out homologous recombination repair, it is necessary to import donor DNA and ensure that it can be accurately expressed. In this way, it can better help the bacteria to carry out homologous recombination repair. Therefore, in order to ensure the correct expression of donor DNA during the transcription process, the T1 terminator is designed to ensure the correct expression of the target gene. In order to improve the CRISPR-MAD7 (cas12a) system in Corynebacterium glutamicum.


Source

Transcription terminator T1 from the E. coli rrnB gene

References